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Validation of MR analysis results in mice. (A,B) Oil <t>Red</t> <t>O</t> <t>staining</t> of the full-length mouse aorta ( n = 5, mean ± standard deviation). (C–F) Serum levels of triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in mice ( n = 5, mean ± standard deviation). (G) Serum levels of total NO in mice ( n = 5, mean ± standard deviation). (H) Relative levels of l -Arg in mouse serum ( n = 5, mean ± standard deviation). (I,J) Relative expression of ARG1 protein in the mouse aorta ( n = 3, mean ± standard deviation). (K) Hematoxylin and eosin staining of the mouse aorta.
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oil red o staining solution  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
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Validation of MR analysis results in mice. (A,B) Oil <t>Red</t> <t>O</t> <t>staining</t> of the full-length mouse aorta ( n = 5, mean ± standard deviation). (C–F) Serum levels of triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in mice ( n = 5, mean ± standard deviation). (G) Serum levels of total NO in mice ( n = 5, mean ± standard deviation). (H) Relative levels of l -Arg in mouse serum ( n = 5, mean ± standard deviation). (I,J) Relative expression of ARG1 protein in the mouse aorta ( n = 3, mean ± standard deviation). (K) Hematoxylin and eosin staining of the mouse aorta.
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Validation of MR analysis results in mice. (A,B) Oil Red O staining of the full-length mouse aorta ( n = 5, mean ± standard deviation). (C–F) Serum levels of triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in mice ( n = 5, mean ± standard deviation). (G) Serum levels of total NO in mice ( n = 5, mean ± standard deviation). (H) Relative levels of l -Arg in mouse serum ( n = 5, mean ± standard deviation). (I,J) Relative expression of ARG1 protein in the mouse aorta ( n = 3, mean ± standard deviation). (K) Hematoxylin and eosin staining of the mouse aorta.

Journal: ACS Omega

Article Title: Gualou-Xiebai-Baijiu Decoction Ameliorates Atherosclerosis by Inhibiting the ARG1/ l ‑Arg/NO Pathway: A Study Based on Network Pharmacology

doi: 10.1021/acsomega.5c06829

Figure Lengend Snippet: Validation of MR analysis results in mice. (A,B) Oil Red O staining of the full-length mouse aorta ( n = 5, mean ± standard deviation). (C–F) Serum levels of triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in mice ( n = 5, mean ± standard deviation). (G) Serum levels of total NO in mice ( n = 5, mean ± standard deviation). (H) Relative levels of l -Arg in mouse serum ( n = 5, mean ± standard deviation). (I,J) Relative expression of ARG1 protein in the mouse aorta ( n = 3, mean ± standard deviation). (K) Hematoxylin and eosin staining of the mouse aorta.

Article Snippet: Reagents used in this study encompassed 4% paraformaldehyde universal tissue fixative (BL539A; Labgic, Beijing, China), saturated Oil Red O staining solutions (G1015; Servicebio, Wuhan, China), and hematoxylin and eosin staining solutions (G1076; Servicebio).

Techniques: Biomarker Discovery, Staining, Standard Deviation, Expressing